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Flow cytometry is a technique that allows the analysis of physical and chemical properties of suspended particles. Providing data for each particle individually, it allows the analysis of thousands of particles in a few minutes. Applied to the 'nucleus' particle, this technique allows the determination of genome sizes, identification of ploidy levels and characterization of phases of the nuclear cycle.

Goals

This course differs from other courses by focusing on a concrete set of applications (cytogenomics) for which there will be no analogous courses. Restricting it to plants and fungi, the course allows potential candidates working in these areas to have differentiation and greater identification with the scope of the course. This course differs from others in that it seeks to maintain a balance between the technical/operational and scientific components (applied to plants and fungi), including the study of cases proposed by the trainees (to which around 40% of the course time is allocated). As this is an emerging methodology and somewhat unique equipment, the added value that the trainee will acquire will be the acquisition of theoretical and practical tools that will allow him to design studies/assays where cytogenomics also becomes a variable under analysis. Especially in the case of 2nd and 3rd cycle students, the course will also allow you to add a differentiating topic to your CV.

  • Contents

    Session 1 (2h) Introduction: presentation of the course; practical and logistical aspects; introduction to flow cytometry (theoretical foundations); presentation of trainees, encouraging the presentation of case studies

Recipients

Scientific community interested in Plant Breeding, Botany, Mycology, Genetics and Ecology; 2nd and 3rd cycle students; professionals in applied areas.

Requirements

Open to all interested parties, no prior knowledge is necessary.

Applications

Opening - April 14, 2023

Closing - June 16, 2023


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Coordination and Trainers

- Pedro Talhinhas

 

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